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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/01/2018 |
Data da última atualização: |
27/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. |
Afiliação: |
Helena F. R. de A. Saraiva, UFF; Ribrio I. T. P. Batista, UFF; Vivian A. P. Alfradique, UFF; Pedro H. N. Pinto, UFF; Lilian S. Ribeiro, UFF; Clara S. Oliveira, UFF; Joanna M. G. de Souza-Fabjan, UFF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Z. Brandão, UFF. |
Título: |
L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, jan. 2018. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C... Mostrar Tudo |
Palavras-Chave: |
In vivo embryo production; Santa ines. |
Thesagro: |
Ovis Aries. |
Thesaurus Nal: |
cryopreservation; gene expression. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02970naa a2200289 a 4500 001 2085659 005 2023-01-27 008 2018 bl uuuu u00u1 u #d 100 1 $aSARAIVA, H. F. R. de A. 245 $aL-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression.$h[electronic resource] 260 $c2018 520 $aAbstract: L-carnitine is an antioxidant and ?-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. 650 $acryopreservation 650 $agene expression 650 $aOvis Aries 653 $aIn vivo embryo production 653 $aSanta ines 700 1 $aBATISTA, R. I. T. P. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aPINTO, P. H. N. 700 1 $aRIBEIRO, L. S. 700 1 $aOLIVEIRA, C. S. 700 1 $aSOUZA-FABJAN, J. M. G. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDAO, F. Z. 773 $tTheriogenology$gv. 105, p. 150-157, jan. 2018.
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
07/02/2020 |
Data da última atualização: |
17/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
FERREIRA-NETO, J. R. C.; SILVA, M. D. da; BENKO-ISEPPON, A. M.; PANDOLFI, V.; BINNECK, E.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; KIDO, E. A. |
Afiliação: |
José Ribamar Costa Ferreira-Neto, UFPE, Recife, PE; Manassés Daniel da Silva, UFPE, Recife, PE; Ana Maria Benko-Iseppon, UFPE, Recife, PE; Valesca Pandolfi, UFPE, Recife, PE; ELISEU BINNECK, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; UFPE, Recife, PE. |
Título: |
Inositol phosphates and Raffinose family oligosaccharides pathways: Structural genomics and transcriptomics in soybean under root dehydration |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Plant Gene, v. 20, 100202, 2019. |
Páginas: |
13 p. |
Idioma: |
Inglês |
Thesagro: |
Glycine Max; Seca; Stress. |
Thesaurus NAL: |
Abiotic stress; Fabaceae; Heat stress. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 00812naa a2200277 a 4500 001 2120041 005 2020-12-17 008 2019 bl uuuu u00u1 u #d 100 1 $aFERREIRA-NETO, J. R. C. 245 $aInositol phosphates and Raffinose family oligosaccharides pathways$bStructural genomics and transcriptomics in soybean under root dehydration$h[electronic resource] 260 $c2019 300 $a13 p. 650 $aAbiotic stress 650 $aFabaceae 650 $aHeat stress 650 $aGlycine Max 650 $aSeca 650 $aStress 700 1 $aSILVA, M. D. da 700 1 $aBENKO-ISEPPON, A. M. 700 1 $aPANDOLFI, V. 700 1 $aBINNECK, E. 700 1 $aNEPOMUCENO, A. L. 700 1 $aABDELNOOR, R. V. 700 1 $aKIDO, E. A. 773 $tPlant Gene$gv. 20, 100202, 2019.
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